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Subjects
Class 11BiotechnologyPrinciples of BiotechnologyLigase EnzymesEasy
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Which is the correct chemical pair in a ligase reaction?

Answer and explanation

Correct answer: 5 prime phosphate and 3 prime hydroxyl

DNA ligase forms a phosphodiester bond between 5 prime phosphate and 3 prime hydroxyl. Remember this as the chemical basis.

Related tags

5 Prime Phosphate3 Prime HydroxylLigationLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq
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10 more MCQs
  1. 1What is the function of DNA ligase enzyme?
  2. 2Why is DNA ligase considered necessary in genetic engineering?
  3. 3Why is DNA ligase needed after restriction enzymes?
  4. 4If ligase is not added what problem will occur with cut vector and insert?
  5. 5After DNA cutting by a restriction enzyme who forms the permanent bond?
  6. 6What is the main function of a ligase enzyme?
  7. 7Which type of bond is formed by DNA ligase?
  8. 8Why is ligase used in recombinant DNA technology?
  9. 9What can ligase enzyme be called in simple language?
  10. 10What is the correct difference between restriction enzyme and DNA ligase?
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