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What type of new bond forms between DNA ends in a ligase reaction?

Answer and explanation

Correct answer: covalent phosphodiester bond

DNA ligase forms a covalent phosphodiester bond between the 3′-OH end and the 5′-phosphate end of a DNA strand. This permanently seals a nick in the sugar-phosphate backbone. Hydrogen bonds occur between complementary bases, so they do not seal DNA ends. Exam tip: Associate DNA ligase with formation of phosphodiester bonds in the DNA backbone.

Related tags

Dna LigasePhosphodiester BondDna BackboneRecombinant DnaBiotechnology EnzymesLigase EnzymesPrinciples Of BiotechnologyBiotechnology

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