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Without ligase what will be the main problem in cloning?लाइगेज के बिना cloning में क्या मुख्य समस्या होगी?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Cut vector and insert will not join permanentlycut vector और insert स्थायी रूप से नहीं जुड़ेंगे
Restriction enzyme can cut but ligase performs permanent joining. Therefore ligase is essential in cloning.Restriction enzyme cutting कर सकता है लेकिन permanent joining ligase करता है। इसलिए ligase cloning में आवश्यक है।
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