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Class 11 Biotechnology Principles of Biotechnology Ligase EnzymesEasy

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How can alkaline phosphatase help reduce self-ligation in cloning?

Answer and explanation

Correct answer: By removing 5 prime phosphate from cut vector

Removing 5 prime phosphate from vector ends can reduce self-ligation. In exams link phosphate removal with ligation control.

Related tags

Alkaline Phosphatase Self Ligation 5 Prime Phosphate Ligase Enzymes Principles Of Biotechnology Biotechnology Class 11 Mcq

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