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Which energy source is generally used by T4 DNA ligase?

Answer and explanation

Correct answer: ATP

T4 DNA ligase uses ATP during formation of a phosphodiester bond between adjacent broken DNA ends. ATP activates the enzyme, enabling it to join the DNA strands. \(NAD^+\) is a cofactor for some bacterial DNA ligases, such as E. coli DNA ligase, but not for T4 DNA ligase. Exam tip: Remember T4 DNA ligase as ATP-dependent, while most bacterial DNA ligases are \(NAD^+\)-dependent.

Related tags

T4 Dna LigaseAtpDna LigationBiotechnologyRecombinant DnaLigase EnzymesPrinciples Of BiotechnologyClass 11 Mcq

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