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On which strand are Okazaki fragments formed?

Answer and explanation

Correct answer: Lagging strand

Direct answer: D, the lagging strand. DNA polymerase can add nucleotides only in the 5′ to 3′ direction. Because the two DNA template strands run antiparallel, one new strand is made continuously toward the replication fork; this is the leading strand. The other new strand must be made away from the fork in short pieces, each begun by an RNA primer. These short pieces are Okazaki fragments, so they form on the lagging strand. DNA ligase later seals the remaining nicks between them, making one continuous strand. Option D is correct. Option A is wrong because the leading strand is synthesized continuously, not as Okazaki fragments. Option B is wrong because RNA may provide primers but is not the strand being described. Option C is wrong because proteins are not DNA replication strands. Memory cue: lagging means delayed and discontinuous; Okazaki fragments belong to lagging.

Related tags

Okazaki FragmentsLagging StrandDna Replication

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