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Which option correctly states the main function of DNA ligase?

Answer and explanation

Correct answer: Joining DNA fragments by forming phosphodiester bonds

DNA ligase joins DNA fragments by forming phosphodiester bonds in the sugar-phosphate backbone. Therefore, it is used in recombinant DNA technology to join a vector with the desired DNA fragment. Restriction endonucleases cut DNA at specific sequences, whereas helicase unwinds the DNA double helix. Exam tip: remember ligase as the “DNA glue” enzyme.

Related tags

Dna LigaseRecombinant DnaPhosphodiester BondRestriction EnzymeBiotechnology EnzymesLigase EnzymesPrinciples Of BiotechnologyBiotechnology

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