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What is the best one-line definition in the topic Ligase Enzymes?

Answer and explanation

Correct answer: Enzymes that join DNA ends with help of energy

The direct answer is option A: ligase enzymes join DNA ends with the help of energy. DNA has a sugar-phosphate backbone, and a complete joining requires formation of a phosphodiester bond between neighbouring nucleotides. DNA ligase catalyses this bond-forming reaction and uses chemical energy supplied by the reaction system. This is why it is used in DNA repair, replication, and recombinant-DNA cloning. Option A is correct because it states both the action, joining DNA ends, and the need for energy. Option B is wrong because cutting DNA at a particular recognition site is the function of a restriction endonuclease. Option C is wrong because breaking proteins into amino acids is done by proteases, not ligase. Option D is wrong because converting water into oxygen is not the definition of ligase and involves unrelated biological processes. Exam cue: ligase joins; restriction enzyme cuts.

Related tags

DefinitionLigase EnzymesDna Ends

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