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What type of action does ligase enzyme perform?

Answer and explanation

Correct answer: bond formation

Direct answer: A, bond formation. Ligase enzymes join molecules or parts of the same molecule by catalyzing bond formation. In DNA work, DNA ligase joins adjacent DNA ends and forms phosphodiester bonds in the sugar-phosphate backbone. The reasoning is: DNA ends are aligned, the enzyme positions the reacting groups, energy is used as required, and a covalent bond is formed. Option A is correct because it names the defining action. Option B is wrong because random bond breaking is not ligase action; controlled cleavage is associated with nucleases or restriction enzymes. Option C is wrong because light production is unrelated to ligase chemistry. Option D is wrong because water evaporation is a physical change, not enzyme catalysis. Do not confuse ligase with lyase or nuclease: ligase joins, while nucleases cut nucleic acids. Memory cue: the word ligase sounds like link, so it links pieces.

Related tags

Bond FormationDna LigaseEnzyme Action

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