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Which option gives an incorrect role of ligase?

Answer and explanation

Correct answer: Cutting DNA at a restriction site

DNA ligase forms phosphodiester bonds to join broken DNA ends or seal nicks. Hence, it joins Okazaki fragments and joins a vector with an insert during recombinant DNA formation. Cutting DNA at a restriction site is performed by a restriction endonuclease, not by ligase. Exam tip: remember restriction enzyme for cutting and ligase for joining.

Related tags

Dna LigaseRestriction EnzymesRecombinant DnaOkazaki FragmentsDna ReplicationLigase EnzymesPrinciples Of BiotechnologyBiotechnology

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