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Which is the most appropriate substrate for DNA ligase?

Answer and explanation

Correct answer: Nicked DNA

An enzyme’s substrate is the molecule or structure on which it acts. DNA ligase recognises DNA ends that are already close together but have a discontinuity in one sugar-phosphate strand; this structure is called nicked DNA. The enzyme seals the nick by forming the missing phosphodiester bond, thereby restoring a continuous backbone. Therefore option A is the best answer. Glucose is a carbohydrate, fatty acid is a lipid component, and chlorophyll is a photosynthetic pigment. None provides adjacent DNA nucleotide ends or the nucleic-acid backbone required for ligase activity. Ligase joins pre-existing DNA structures rather than metabolising ordinary cellular nutrients or pigments.

Related tags

Nicked DnaEnzyme SubstrateDna LigaseLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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