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Which option gives the correct relation between ligase and the phosphodiester bond?

Answer and explanation

Correct answer: Ligase forms the bond

DNA ligase is a joining enzyme whose central action is sealing a discontinuity in a DNA backbone. It aligns a 3′ hydroxyl end with a neighbouring 5′ phosphate end and, using suitable chemical energy, catalyses formation of the phosphodiester bond between them. Thus option A states the correct relation. The enzyme does not merely break this bond; its characteristic role in replication, repair, and recombinant-DNA construction is to create the missing linkage. Colouring a bond is a staining or visualisation concept, not catalysis, and claiming no relation directly contradicts the known function of ligase. The answer therefore follows from the enzyme’s substrate and reaction.

Related tags

Phosphodiester BondLigase FunctionDna BackboneLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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