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Which option is a correct statement for ligase?

Answer and explanation

Correct answer: It can seal DNA nicks

The direct answer is option A: ligase can seal DNA nicks. A DNA nick is a small break in one strand of the DNA sugar-phosphate backbone, while the two parts are still held together by the other strand. DNA ligase uses energy to form a phosphodiester bond and close this break. This helps complete DNA replication, repair damaged DNA, and join fragments during cloning. Option A is correct because sealing a nick is a main function of DNA ligase. Option B is wrong because DNA is not changed into sugar; its backbone already contains sugar, and this conversion is not ligase activity. Option C is wrong because cutting DNA at specific recognition sequences is mainly done by restriction endonucleases. Option D is wrong because ligase does not work only on lipids; DNA ligase acts on DNA ends. Memory cue: ligase is the DNA “glue,” whereas a restriction enzyme is the DNA “scissors.”

Related tags

Dna NicksLigaseReplication Repair

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