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Which option is an incorrect condition for ligase reaction?

Answer and explanation

Correct answer: completely boiled enzyme

DNA ligase joins DNA ends by forming phosphodiester bonds, so the enzyme must be active. A completely boiled enzyme is usually denatured and loses its catalytic activity; therefore, it is an incorrect condition for a ligase reaction. Compatible DNA ends and an energy source such as ATP support ligation. Exam tip: remember that excessive heat denatures most enzymes and prevents them from functioning.

Related tags

Dna LigaseLigation ReactionEnzyme DenaturationRecombinant DnaBiotechnology PrinciplesLigase EnzymesPrinciples Of BiotechnologyBiotechnology

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