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Why is it important to protect ligase from heat?

Answer and explanation

Correct answer: Excess heat can denature ligase and cause it to lose its enzymatic activity

Ligase is a protein enzyme that helps form phosphodiester bonds between broken DNA ends. Excess heat can denature its three-dimensional structure, altering the active site and reducing or destroying enzyme activity. A restriction enzyme cuts DNA, whereas ligase joins DNA fragments, so option C is incorrect. Exam tip: For protein enzymes, excessive temperature is commonly associated with denaturation and loss of activity.

Related tags

Dna LigaseEnzyme DenaturationHeat SensitivityBiotechnologyRestriction EnzymesLigase EnzymesPrinciples Of BiotechnologyClass 11 Mcq

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