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Which option gives the correct substrate of ligase enzyme?

Answer and explanation

Correct answer: Cut or nicked DNA ends

DNA ligase acts at cut DNA fragments or nicks in DNA. Using energy from ATP, it forms a phosphodiester bond in the sugar-phosphate backbone and joins the broken ends. Replicating single-stranded DNA is not its direct substrate. Exam tip: remember ligase as the enzyme that joins DNA fragments.

Related tags

Dna LigaseLigase EnzymeNicked DnaDna EndsPhosphodiester BondBiotechnologyLigase EnzymesPrinciples Of Biotechnology

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