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Which use of ligase is related to DNA repair?

Answer and explanation

Correct answer: Sealing a single-strand nick

DNA repair often leaves a discontinuity, called a single-strand nick, in an otherwise correctly paired DNA molecule. The repair pathway fills or restores the missing nucleotide region, but the final interruption in the sugar-phosphate backbone must be sealed. DNA ligase performs this final sealing by forming the missing phosphodiester bond. Therefore option A is correct and represents a genuine repair-related use. Protein folding is assisted by chaperones and is not the role of ligase. Lipid digestion is carried out by lipases, while colouring a cell wall is a laboratory staining action, not a DNA-repair reaction. Ligase repairs structural breaks in nucleic-acid backbones.

Related tags

Dna RepairSingle-Strand NickDna LigaseLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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