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If insert and vector ends lack 5 prime phosphate what happens to ligation?यदि insert और vector के सिरों में 5 prime phosphate नहीं है तो ligation पर क्या असर होगा?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Ligation may be affectedलाइगेशन प्रभावित हो सकती है
5 prime phosphate is needed to form a phosphodiester bond. Its absence can reduce ligation efficiency.5 prime phosphate phosphodiester bond बनाने के लिए जरूरी होता है। इसकी कमी ligation efficiency घटा सकती है।
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