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What is the phosphodiester bond made by DNA ligase a part of?

Answer and explanation

Correct answer: Sugar-phosphate backbone of DNA

The governing concept is that DNA nucleotides are connected through a sugar-phosphate backbone. A phosphodiester bond links the 3′ hydroxyl group of one deoxyribose sugar to the 5′ phosphate group of the next nucleotide. DNA ligase catalyses formation of this covalent bond when it joins adjacent DNA ends or seals a nick. Therefore, option A is correct: the bond becomes part of the DNA sugar-phosphate backbone. A cell wall is a structural layer outside some cells, a fat layer is made mainly of lipids, and a ribosome surface is not the structure being repaired or joined by ligase. Ligase joins DNA; it does not make any of those distractor structures.

Related tags

Phosphodiester BondDna BackboneDna LigaseLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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