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In Class 11 biotechnology, DNA ligase is most closely associated with which process?

Answer and explanation

Correct answer: Joining DNA fragments

DNA ligase is commonly associated with joining DNA fragments in recombinant-DNA technology. After a vector and an insert have been cut and aligned, ligase seals the breaks in their sugar-phosphate backbones and forms a recombinant DNA molecule. It also joins Okazaki fragments during normal DNA replication, but the central biotechnology association in cloning is DNA-fragment joining. Option A is therefore correct. Respiratory gas exchange is a physiological function of organs, transpiration is water loss from leaves, and bone formation is a process of animal tissue development. None of these processes describes the molecular action of DNA ligase.

Related tags

Dna LigaseCloningDna FragmentsLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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