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Class 11 Biotechnology Principles of Biotechnology Ligase EnzymesEasy

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Why is an energy source added in ligation buffer?

Answer and explanation

Correct answer: For bond-forming action of ligase

Ligase needs energy to make a new phosphodiester bond. Therefore ligation buffer contains an energy source.

Related tags

Ligation Buffer Energy Source Dna Ligase Ligase Enzymes Principles Of Biotechnology Biotechnology Class 11 Mcq

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