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What does ligase not do?

Answer and explanation

Correct answer: Cutting a specific sequence in DNA

The direct answer is D: ligase does not cut a specific DNA sequence. DNA ligase is a joining enzyme. It joins DNA ends and seals breaks, called nicks, in the sugar-phosphate backbone by forming phosphodiester bonds. During DNA replication, it also joins Okazaki fragments on the lagging strand after the separate pieces have been made. Option A is therefore a true ligase function. Option B is also true because sealing a nick is exactly a repair or joining action performed by ligase. Option C is true because ligase connects Okazaki fragments to make a continuous strand. Option D is correct as the exception: recognizing and cutting a particular DNA sequence is the role of a restriction endonuclease, not ligase. The word “not” is the key in this question. Memory cue: ligase is like molecular glue; restriction enzyme is the molecular scissors.

Related tags

Ligase FunctionNot CuttingRestriction Enzyme

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