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Who makes temporary base pairing between cut DNA fragments permanent?

Answer and explanation

Correct answer: DNA ligase

Direct answer: A, DNA ligase. When DNA is cut, sticky ends may first meet through temporary complementary base pairing. These hydrogen-bond interactions hold the fragments near each other, but they do not make the sugar-phosphate backbones continuous. DNA ligase completes the repair by catalyzing formation of phosphodiester bonds between the proper 3′ hydroxyl and 5′ phosphate groups. This seals the nick and makes the recombinant DNA strand stable. Option A is correct. Option B, chlorophyll, captures light energy in plants and does not join DNA. Option C, a ribosome, helps translate mRNA into protein and does not seal DNA. Option D, a fatty acid, is a lipid building block and is not a DNA enzyme. The same ligase principle operates in cloning and during DNA replication, where separate pieces must be joined. Memory cue: base pairing brings DNA together; ligase seals the backbone.

Related tags

Base PairingDna LigaseSticky Ends

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