Which bond makes the permanent DNA join formed by ligase strong and stable?Ligase द्वारा बनाए गए स्थायी DNA join को मजबूत और stable कौन-सा bond बनाता है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: Phosphodiester bondPhosphodiester bond
DNA ligase completes the sugar-phosphate backbone by catalysing formation of a phosphodiester bond between a 3′-hydroxyl group and a 5′-phosphate group on adjacent DNA ends. This is a covalent bond, so it creates a stable, permanent connection rather than merely holding the strands together through temporary base pairing or weak molecular forces. Option A is therefore correct. Hydrogen bonds between complementary bases help hold the two DNA strands together, but they are not the new backbone bond made by ligase. The other listed ‘bonds’ are not recognised chemical bonds involved in DNA ligation and are biologically irrelevant.DNA ligase DNA की sugar-phosphate backbone को पूरा करते हुए adjacent DNA ends के 3′-hydroxyl group और 5′-phosphate group के बीच phosphodiester bond बनाता है। यह एक मजबूत covalent bond होता है, इसलिए केवल temporary base pairing या कमजोर molecular forces के बजाय DNA fragments का स्थायी connection बनता है। अतः option A सही है। Complementary bases के बीच hydrogen bonds दोनों DNA strands को साथ रखने में सहायता करते हैं, लेकिन ligase द्वारा नया backbone bond hydrogen bond नहीं होता। Weak smell bond, colour bond और fat bond मान्य रासायनिक bonds नहीं हैं और DNA ligation में उनकी कोई भूमिका नहीं होती।
Student feedback
Was this question useful?
👍 0 Helpful 👎 0 Not helpful
Yes 0% No 0%
0 responsesStudent Reviews
No published reviews yet.
