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DNA ligase forms a bond between which parts?

Answer and explanation

Correct answer: Between the 3′-OH and 5′-phosphate ends of adjacent DNA nucleotides

DNA ligase forms a phosphodiester bond between the 3′-OH end and the 5′-phosphate end of adjacent DNA nucleotides. This seals a nick or break in the sugar-phosphate backbone. A peptide bond is formed between amino acids, so option B is not correct. Exam tip: remember ligase as the enzyme that joins broken DNA ends.

Related tags

Dna LigasePhosphodiester BondDna ReplicationRecombinant DnaBiotechnology EnzymesLigase EnzymesPrinciples Of BiotechnologyBiotechnology

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