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After DNA cutting by a restriction enzyme who forms the permanent bond?

Answer and explanation

Correct answer: DNA ligase

Restriction enzymes and DNA ligase perform different steps in recombinant-DNA construction. A restriction enzyme cleaves DNA at a particular recognition site, producing fragments with compatible or blunt ends. DNA ligase then seals the adjacent DNA strands by catalysing formation of phosphodiester bonds in the sugar-phosphate backbone. This creates a stable recombinant DNA molecule. A ribosome translates messenger RNA into protein, chlorophyll captures light during photosynthesis, and glucose is a carbohydrate; none of these joins DNA fragments. Therefore option A, DNA ligase, is the only correct answer.

Related tags

Dna LigasePhosphodiester BondDna JoiningRestriction EnzymeLigase EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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