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What is the main difference between blunt ends and sticky ends?

Answer and explanation

Correct answer: Sticky ends have single-stranded overhangs

The direct answer is A: sticky ends have single-stranded overhangs. DNA usually has two complementary strands. Some restriction enzymes cut the two strands at different positions, leaving a short unpaired extension. This extension can pair with a complementary extension on another DNA fragment; this is why it is called sticky. A blunt end is cut straight across both strands, so it has no single-stranded overhang. Option A is correct because it describes the structural difference exactly. Option B is wrong because blunt ends are DNA ends, not proteins. Option C is wrong because sticky ends are still parts of DNA. Option D is wrong because the two types differ in their structure and in how easily they join. Memory cue: sticky means an exposed matching overhang; blunt means a straight cut.

Related tags

Blunt EndsSticky EndsDna Fragments

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