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After inserting a foreign gene into a host, what should be checked immediately?

Answer and explanation

Correct answer: क्या जीन सफलतापूर्वक प्रविष्ट हुआ और अभिव्यक्त हो रहा है

Successful gene insertion and gene expression are separate issues. A DNA construct may fail to enter the host, may be lost, may be inserted in the wrong orientation or may remain silent even after entering the cell. Therefore, screening or verification should first establish that the intended recombinant cells contain the foreign gene; an expression test can then confirm production of the expected RNA or protein. Option A is the only scientifically relevant choice. Water colour, soil weight and the status of a light source do not indicate whether transformation or expression has occurred.

Related tags

Gene-InsertionGene-ExpressionScreeningGenetic EngineeringPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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