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What is the main function of DNA polymerase during the extension step of PCR?

Answer and explanation

Correct answer: To synthesize a new strand complementary to the template DNA

During PCR extension, DNA polymerase adds nucleotides from the primer’s 3′ end to synthesize a new DNA strand complementary to the template. Strand separation occurs during denaturation, primer binding occurs during annealing, and cutting at specific sites is performed by restriction enzymes. Exam tip: The extension step is commonly carried out at about 72°C, suitable for Taq DNA polymerase.

Related tags

PcrExtensionDna PolymeraseGenetic EngineeringMolecular BiologyPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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