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What is the advantage of sticky ends?

Answer and explanation

Correct answer: They easily join with complementary DNA ends

The direct answer is A: sticky ends easily join complementary DNA ends. A restriction enzyme can cut two DNA molecules in a staggered way, leaving short single-stranded overhangs. These overhangs are called sticky ends because they can temporarily pair through complementary bases: A pairs with T, and G pairs with C. Step by step: the same or compatible restriction enzyme makes matching cuts; the exposed bases pair by hydrogen bonds; DNA ligase seals the sugar-phosphate backbone; recombinant DNA is formed. Option A is correct because complementary ends fit together and make joining easier and more specific. Option B is wrong because sticky ends do not destroy DNA; restriction enzymes make the cuts, while sticky ends help joining. Option C is wrong because they do not heat the cell. Option D is wrong because they do not inactivate proteins. Memory cue: sticky ends “stick” by complementary base pairing before ligase seals them.

Related tags

Sticky EndsComplementary BasesRdna

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