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What is the primary function of the DNA ligase enzyme in genetic engineering?

Answer and explanation

Correct answer: Joining DNA fragments by forming phosphodiester bonds between them

DNA ligase joins broken or separate DNA fragments by forming phosphodiester bonds between them. This is why it is used to construct recombinant DNA molecules. Option B is incorrect because restriction endonucleases cut DNA at specific recognition sites. Exam tip: Remember ligase as the biological ‘glue’ that seals DNA fragments.

Related tags

Dna LigaseGenetic EngineeringRecombinant DnaDna EnzymesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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