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What can be useful to reduce expression burden in a competent host?

Answer and explanation

Correct answer: Controlled promoter

The governing concept is regulation of heterologous gene expression. A foreign gene can consume cellular energy, nucleotides, amino acids, ribosomes, and folding capacity when it is expressed too strongly or continuously. A controlled promoter allows expression to be induced when needed, adjusted to a suitable level, or kept low during growth. This can reduce metabolic burden and improve host viability and plasmid stability. Option A is therefore correct. Unlimited expression generally increases stress, while removing the vector removes the expression construct rather than regulating it. Removing nutrients would harm growth and is not a controlled solution to expression burden.

Related tags

Expression BurdenControlled PromoterCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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