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What happens if a wrong antibiotic plate is used in competent host work?

Answer and explanation

Correct answer: Correct transformants may fail to grow or may be missed

After transformation, the antibiotic plate must match the selectable marker present on the plasmid. For example, transformants carrying an ampicillin-resistance plasmid may not grow on a kanamycin plate, so genuine transformants can be missed. Option B is incorrect because antibiotic selection only selects resistant cells; it does not fully confirm every colony as a transformant. Exam tip: always match the plasmid resistance gene with the antibiotic used on the plate.

Related tags

Competent HostTransformationAntibiotic SelectionSelectable MarkerPlasmid ResistancePrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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