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What happens if heat shock is too strong in a competent host?

Answer and explanation

Correct answer: Cell survival can decrease

Heat shock is applied at a controlled temperature and duration so that the cell membrane temporarily becomes favourable for DNA entry. If it is too strong, cells may be damaged, reducing their survival and transformation efficiency. DNA uptake does not always increase; excessive heat is harmful to cells. Exam tip: remember that an optimum heat shock aids DNA uptake, whereas excessive heat causes cell damage.

Related tags

Competent HostHeat ShockTransformationCell SurvivalBiotechnologyPrinciples Of BiotechnologyClass 11 Mcq

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