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Why is a probe used in library screening?

Answer and explanation

Correct answer: To find a clone with complementary sequence

The direct answer is A: a probe is used to find a clone containing a complementary sequence. A nucleic-acid probe is a labelled single-stranded DNA or RNA fragment designed to match a target sequence. Under suitable conditions, complementary bases pair with one another, so the probe binds to a clone or its DNA when the desired sequence is present. The label then produces a detectable signal, helping the researcher identify the correct colony or library member among many candidates. Option A is correct because complementary binding is the central principle. Option B is wrong because a probe is not used to burn a host. Option C is wrong because it does not eat agar. Option D is wrong because it does not increase protein smell. The memory cue is: probe means “find the matching sequence.”

Related tags

Library ScreeningProbeComplementary Sequence

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