When can periplasmic targeting be useful in bacterial expression?Periplasmic targeting bacterial expression में कब useful हो सकता है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: In folding or disulfide formation of some proteinsकुछ proteins की folding या disulfide formation में
Periplasmic targeting directs a recombinant protein through the bacterial inner membrane into the periplasmic space. This compartment can provide conditions that are different from the cytoplasm, including an environment that may support the folding, processing, or formation of disulfide bonds in some proteins. Hence, option A is correct, although not every protein benefits and export capacity can become limiting. Periplasmic targeting is not primarily used to stop DNA uptake, convert the host into water, or remove a selectable marker. It is an expression strategy chosen when location and protein maturation are important.Periplasmic targeting में recombinant protein को bacterial inner membrane के पार periplasmic space में भेजा जाता है। यह compartment cytoplasm से अलग परिस्थितियाँ प्रदान कर सकता है और कुछ proteins के folding, processing तथा disulfide bonds बनने में सहायता कर सकता है। इसलिए विकल्प A सही है, हालांकि हर protein को इसका लाभ नहीं मिलता और export capacity सीमित हो सकती है। Periplasmic targeting का मुख्य उद्देश्य DNA uptake रोकना, host को पानी में बदलना या selectable marker हटाना नहीं है। यह expression strategy तब चुनी जाती है जब protein का cellular location और maturation उसकी activity या recovery के लिए महत्वपूर्ण हो।
Student feedback
Was this question useful?
👍 0 Helpful 👎 0 Not helpful
Yes 0% No 0%
0 responsesStudent Reviews
No published reviews yet.
