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What problem occurs if contamination enters a competent host culture?

Answer and explanation

Correct answer: Wrong colonies and wrong interpretation may occur

Contamination introduces unwanted organisms or material into a culture, so colonies observed on a plate may not all originate from the intended competent host and transformation experiment. Consequently, colony counts, selectable-marker results, plasmid analysis, and later interpretation may become unreliable. Option A is correct because contamination can produce unexpected colonies or obscure the true result. It does not automatically purify DNA, improve the host, or strengthen a marker. Aseptic handling, sterile media and instruments, appropriate positive and negative controls, and examination of colony morphology help detect or prevent contamination. If contamination is suspected, the result should not be accepted without repeating the experiment.

Related tags

ContaminationCompetent HostAseptic TechniquePrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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