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Why can excessive DNA amount be a problem in a competent host?

Answer and explanation

Correct answer: Cells may be stressed and transformation efficiency may decrease

Adding too much DNA to competent host cells can increase osmotic or physical stress and may make DNA uptake less efficient. Therefore, an appropriate DNA amount is used for transformation. Option B is incorrect because competence does not guarantee transformation of every cell. Exam tip: Remember that transformation efficiency depends on using optimal amounts of both competent cells and DNA.

Related tags

Competent HostDna TransformationTransformation EfficiencyCell StressBiotechnology PrinciplesPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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