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What can happen if DNA purity is low in transformation?

Answer and explanation

Correct answer: Transformation efficiency may decrease because DNA uptake or cell survival can decrease

Salts, phenol, ethanol, proteins, or other contaminants in DNA can damage competent cells and reduce plasmid DNA uptake. Hence, transformation efficiency and survival of transformed cells may decrease. Option B is incorrect because impurities generally interfere with transformation rather than activating cells. Exam tip: purified, contaminant-free DNA gives more reliable transformation results.

Related tags

TransformationDna PurityCompetent CellsTransformation EfficiencyPlasmid UptakeCompetent HostPrinciples Of BiotechnologyBiotechnology

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