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When can choosing a mammalian host cell be appropriate?

Answer and explanation

Correct answer: Complex eukaryotic protein processing is needed

Host selection is governed by the biological processing required for the final product. Mammalian cells possess eukaryotic machinery that can perform complex folding, disulfide-bond formation, trafficking and many post-translational modifications that may be important for a mammalian therapeutic protein. Therefore option A is correct when authentic or complex eukaryotic processing is needed. A mammalian system is not chosen merely to obtain plasmid copies or an agar colony; microbial hosts are often more convenient for those purposes. Drying the host is not a valid production objective. Mammalian culture can be slower, more expensive and technically demanding, so the required product quality must justify the choice.

Related tags

Mammalian HostComplex ProteinProtein ProcessingCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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