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Why is gentle mixing better in transformation?

Answer and explanation

Correct answer: To protect competent cells from damage

During transformation, competent cells—especially chemically prepared cells—may be fragile. Vigorous vortexing or pipetting can damage their membranes, reducing cell viability and their ability to take up DNA. Gentle mixing distributes the DNA while protecting the cells. Cutting DNA is the function of restriction enzymes, not mixing. Exam tip: link “gentle handling” in transformation with preservation of cell viability and competence.

Related tags

TransformationCompetent CellsCell ViabilityDna UptakeBiotechnology PrinciplesCompetent HostPrinciples Of BiotechnologyBiotechnology

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