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Why is a recovery period kept after transformation in a competent host?

Answer and explanation

Correct answer: To let the cell express the marker and recover from stress

Transformation, whether performed by a chemical or electrical method, can temporarily injure cells and disturb their membrane or physiological state. A recovery period in a suitable non-selective medium allows cells to repair damage, resume metabolism and begin expressing the selectable marker encoded by an acquired plasmid. This expression matters because immediate exposure to antibiotic selection may kill transformed cells before enough resistance protein is produced. Therefore option A is correct. The period does not burn DNA, convert the host into soil, or turn an antibiotic into pigment. The exact recovery conditions depend on the host, vector, transformation method and marker system.

Related tags

Recovery PeriodMarker ExpressionCell StressCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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