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What is the benefit of glycerol in a glycerol stock?

Answer and explanation

Correct answer: Protecting cells from freezing damage

The principal benefit of glycerol in a glycerol stock is cryoprotection. When a cell suspension is stored at a very low temperature, ice formation and concentration changes in the remaining liquid can injure cell membranes and other structures. Glycerol helps reduce this freezing injury, so a larger proportion of cells can survive storage and grow after thawing. Hence, option A is correct. DNA cutting is performed by restriction enzymes, transformation is the process of introducing genetic material into a host, and protein colouring is an analytical or staining procedure unrelated to glycerol stock preservation. The concept being tested is long-term storage of viable cells.

Related tags

Glycerol-StockCryoprotectantCell-SurvivalCell-CultureCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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