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What is the purpose of a negative control?

Answer and explanation

Correct answer: Checking contamination or background growth

A negative control is a reaction or transformation setup in which the test plasmid or other intended experimental factor is deliberately omitted. Its governing concept is baseline comparison: any colony or signal in this control should not be attributed to successful uptake of the target plasmid. If growth appears on a selective plate, it may indicate contamination, incorrect antibiotic concentration, degraded selection medium, or naturally resistant cells. Therefore, option A is correct because the control checks background growth and the cleanliness and validity of the selection system. Option B describes an experimental aim, while options C and D concern DNA synthesis or marker expression and are not purposes of a negative control.

Related tags

Negative ControlContaminationBackground GrowthCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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