Why can protease activity be a problem in an expression host?Expression host में protease activity समस्या क्यों हो सकती है?
Answer and explanationउत्तर और व्याख्या
Correct answer:सही उत्तर: It can degrade recombinant proteinRecombinant protein degrade कर सकती है
Proteases are enzymes that hydrolyse peptide bonds in proteins. If an expression host produces active proteases, they may recognize and digest the recombinant protein during or after its synthesis, especially when the protein is misfolded, exposed, or released during cell disruption. Option A is correct because this degradation lowers intact protein yield and may produce unwanted fragments that complicate purification. A protease-deficient host, optimized culture conditions, rapid processing, or suitable stabilizers can help. Proteases do not generally increase DNA uptake, create water, or manufacture the antibiotic represented by a selectable marker.Proteases ऐसे enzymes हैं जो proteins के peptide bonds को hydrolyse करते हैं। यदि expression host में protease activity अधिक हो, तो ये recombinant protein को उसके बनने के दौरान या cell lysis के बाद तोड़ सकते हैं, विशेषकर जब protein misfolded, exposed या अस्थिर हो। इसलिए विकल्प A सही है, क्योंकि degradation से intact protein की yield घटती है और purification में unwanted fragments आ सकते हैं। Protease-deficient host, उचित culture conditions, तेज processing और stabilizers इस नुकसान को कम कर सकते हैं। Proteases DNA uptake नहीं बढ़ाते, पानी नहीं बनाते और selectable marker के antibiotic को स्वयं नहीं बनाते।
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