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Why is induction timing important?

Answer and explanation

Correct answer: Cells start expression after sufficient growth

Induction activates expression of a recombinant gene, but producing the protein too early can impose a large metabolic burden before the culture has developed enough biomass. Cells may grow slowly, become stressed, or produce less final protein. Option A is correct because inducing after sufficient growth separates biomass formation from the costly production phase and can improve both cell health and yield. Timing is not universal; it depends on the promoter, host, protein toxicity, culture density, and process design. The other choices are biologically unrelated: DNA does not become water, drying harms cells, and marker digestion is not the purpose of induction.

Related tags

Induction TimingGene ExpressionCell GrowthProtein YieldCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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