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Why is leaky expression a problem in a toxic gene?

Answer and explanation

Correct answer: Toxic protein can be made even without inducer

Leaky expression means that a promoter intended to be inducible is not completely silent before the inducer is added. Even a small amount of toxic protein may damage the host, slow its growth, reduce viability, or favor cells that lose the recombinant plasmid. Thus option A is correct: toxic protein can be produced even without deliberate induction. Tight promoter control, a suitable host, low basal transcription, or delayed induction can reduce this problem. The other options do not describe promoter leakage: DNA uptake is not automatically improved, a host does not become water, and marker strength is not the defining issue.

Related tags

Leaky ExpressionToxic GenePromoter ControlBasal TranscriptionCompetent HostPrinciples Of BiotechnologyBiotechnologyClass 11 Mcq

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