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How can spontaneous mutation affect cloning results?

Answer and explanation

Correct answer: The DNA sequence of the insert or vector may change

A spontaneous mutation can alter the DNA sequence of the insert or vector. This may affect the intended gene function, protein product, or stability of the clone. Loss of a selectable marker may occur in some cases, but it is not an inevitable outcome of every spontaneous mutation. Exam tip: confirm a cloned construct by DNA sequencing or restriction analysis.

Related tags

Spontaneous MutationCloningVector SequenceInsert DnaClone VerificationCompetent HostPrinciples Of BiotechnologyBiotechnology

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