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Why does sequencing give final confirmation?

Answer and explanation

Correct answer: It can tell the exact insert sequence

The direct answer is A: sequencing can tell the exact insert sequence. Sequencing determines the order of DNA bases in the cloned region. First, DNA from the clone is prepared. Then a sequencing reaction or sequencing platform produces a read of the bases. The obtained sequence is compared with the expected reference sequence. This comparison can show whether the insert is present, whether its orientation is correct when the relevant region is read, and whether mutations or unwanted changes are present. Therefore A is the strongest confirmation. Option B, plate smell, cannot reveal DNA identity. Option C, changing host colour, is not a dependable way to know the exact sequence. Option D, increasing water amount, has no connection with genetic verification. The memory cue is: screening suggests a clone; sequencing reads and confirms its letters.

Related tags

SequencingInsert IdentityMutation Check

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