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Which analysis is common after miniprep?

Answer and explanation

Correct answer: Restriction digestion or sequencing

The direct answer is A: restriction digestion or sequencing. A miniprep is a method used to isolate plasmid DNA from bacterial cells. After isolation, the important question is whether the plasmid contains the expected insert and whether its arrangement is correct. In restriction digestion, selected enzymes cut DNA at known sites, so the resulting band sizes on a gel can support the expected plasmid pattern. Sequencing reads the DNA bases and can provide a more exact confirmation. Option A is correct because both are normal analyses after plasmid preparation. Option B, water tasting, has no role in identifying DNA. Option C, fat staining, studies lipids rather than plasmid structure. Option D, leaf counting, is unrelated to bacterial plasmids. A useful exam cue is: miniprep gives plasmid DNA; digestion gives a pattern, while sequencing gives base-level confirmation.

Related tags

MiniprepRestriction DigestionSequencing

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